Description
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of myostatin in gallus serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to myostatin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to myostatin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substGalluse solution is added, only those wells that contain myostatin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substGalluse reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentGallusion of myostatin in the samples is then determined by comparing the O.D. of the samples to the standard curve